ORTIZ POLYMER MECHANICS LABORATORY MANUAL Room 12-065 (Chemistry Lab, Phone : (617) 258-6154) Room 13-5037 (Nanomechanics Lab, Phone : (617) 253-8779

10 Collaborator Name: Campus Address: Campus Phone / Email: Home Address: Home Phone / Email: A

11 5. LABORATORY SUPPLIES ORDER FORM

12 ORTIZ LABORATORY SUPPLIES ORDER FORM DATE VENDOR NAME VENDOR TELEPHONE OR URL ITEM NAME ITEM NUMBER PRICE (IF OVER $200 OBTAIN AUTHORIZATIO

13 6. LABORATORY SAFETY PROCEDURES AND TRAINING Manditory : I. Center for Materials Science and Engineering Requirements (CMSE) : I.A. Read the OSHA

14 6.1 The DMSE Safety Primer Index • The DMSE Safety Program • Emergencies and First Aid • Basic Elements of Laboratory Safety • Chemical Hy

15 • Access to a laboratory will not be approved, and UROP proposals will not be signed, until the user has passed the examination and provided an a

16 • Mr. Joseph A. Adario Duties of the Safety Committee include: • The Committee assists the Departmental Safety Officer in formulating policies

17 • After the emergency, promptly report the incident to the MIT Safety Office (phone 3-4736, room E19-207). Dial 100 for: • fire • toxic gas

18 • In case of a clothing fire, the victim should drop to the floor and roll, not run to a safety shower. A fire blanket, if nearby, should be used

19 Conducting experiments • All injuries, accidents, and "near misses" must be reported to the Laboratory Supervisor. An Accident Report m

2 TABLE OF CONTENTS : PAGE ITEM 3 1. Checklist for New Group Members and Collaborators 4 2. Ortiz Lab Phone and Office List 5-7 3. General Laborator

20 • Personal items brought into the laboratory should be limited as much as is practical to those things necessary for the experiment. • Safety l

21 many of these are also available from the Internet ( click here). The MSDS should be obtained from the supplier when new chemicals are purchased.

22 Choice of chemicals: • The use of the following five chemicals is illegal in the City of Cambridge, MA, and must not be brought onto the MIT camp

23 Waste Disposal: • A label indicating "Chemical Waste" with the chemical name and concentration should be placed on each container of ch

24 name(s). In any event, at the end of each workday, the contents of all unlabeled containers should be labeled or are to be considered wastes and p

25 • Always store flammable liquids away from oxidizers. • Be aware that liquids with flash points at and above 100oF may also present a significa

26 • A chemical fume hood is a safety backup for condensers, traps, or other devices that collect vapors and fumes. It should not be used to "d

27 Vapor Detection Odor should not be relied upon as a means of determining that inhalation exposure limits are or are not being exceeded. Whenever t

28 whether flammable or nonflammable, miscible or non miscible with water. Organic solvents should be placed in suitable containers (1 gallon maximum

29 Unknown Waste Chemicals Unknown waste chemicals cannot be accepted for disposal. Disposal contractors cannot accept or ship unknown waste. It is t

3 1. CHECKLIST FOR NEW GROUP MEMBERS AND COLLABORATORS ITEM TO BE COMPLETED DATE COMPLETED 1. Please read this entire laboratory manual. 2. Please

30 Work on electrical devices should be done only after the power has been shut off in such a manner that it cannot be turned on accidentally. Intern

31 Oxygen is removed from the air by liquid nitrogen. Therefore, use liquid nitrogen only in a well-ventilated area so that the ambient oxygen concen

32 7. LABORATORY NOTEBOOK GUIDELINES • Each section has a clear, descriptive heading of the experiments which were performed and detailed writing of

33 8. DESCRIPTION OF LABORATORY EQUIPMENT Molecular Force Probe (MFP) Asylum Research, Inc. : • Brief description of MFP and MFP specifications fro

34 PureLab Plus UV/UF Laboratory Water Purification System (US Filter) AT200 Analytical Balance (Mettler-Toledo) PerpHecT

35 Nanomechanics Software : •IC Adams Nanomechanics Software Optical Microscopes : • Axioskop 20, Zeiss, Inc. with transmitted and ref

36 Micro- and Macro- Mechanical Testing Equipment : • Rheometric Scientific (Polymer Labs) Minimat 2000 Miniature Materials Tester with Environmenta

37 9. MFP SAFETY PRECAUTIONS Ortiz Nanomechanics Lab : 13-5037 1. PHOTODIODE AND LASER KNOBS : Please be sure not to push them beyond their range o

38 10. MULTIMODE AFM SAFETY PRECAUTIONS Ortiz Nanomechanics Lab : 13-5037 I. TIPHOLDERS : •••• Only use the AFM tipholders provided with the Multim

39 boxes, or other components containing electronic parts. Avoid spilling all corrosive fluids on exposed surfaces; otherwise damage will result (e.g

4 2. GENERAL LABORATORY POLICIES 1. Ortiz primary students and postdocs have priority on all laboratory equipment. 2. All new group members and coll

40 Withdraw and then re-engage again. Thermal drift or other reasons may cause the z-center to change gradually with time. It change suddenly when if

41 computer, outputs to the piezo might be at random values (up to +/- 220V). •••• Do not unplug cables to / from energized hardware. Turn OFF first.

42 11. FORCE CURVE DATA CONVERSION (M. Rixman) For data from the DI Multimode AFM 1. Export the raw data as an ASCII file Do this in the Offlin

43 a determined distance from the previous z-position has been traveled. The Z distance between data points may be calculated as follows: lineSam

44 12. INSTRUCTION FOR CONDUCTING STATISTICAL ANALYSIS ON HIGH-RESOLUTION FORCE SPECTROSCOPY ADHESION DATA 1. Open the raw experiment *.PXP datafil

45 13. PROCEDURES FOR IMAGING OF STANDARDS AT ATOMIC-SCALE RESOLUTION USING THE DIGITAL INSTRUMENTS MULTIMODE AFM Ortiz Nanomechanics Laboratory@MIT

46 • use oxide-sharpened Si3N4 probe tips, e.g. Model NPS which has the following specifications (http://www.di).com/products2/NewProbeGuide/ContactM

47 sample is rotated until the atoms are oriented vertically or when the fast scan axis is parallel to the a or b crystallographic axis. The Proporti

48 Figure 1. AFM High-Resolution Contact Mode Image of Mica from A.Belyayev, State Research Institute of Physical Problems & NT-MDT, Moscow, Russ

49 Figure 3. AFM High-Resolution Contact Mode Image of HOPG (*downloaded from : http://www.physics.sfasu.edu/afm/afm.htm)

5 3. ORTIZ LAB PHONE LIST F01 CHEMISTRY LAB : 12-065 , PHONE : 258-6154 NANOMECHANICS LAB : 13-5037 , PHONE : 253-8779 GRAD STUDENT OFFICE : 12-02

50 Figure 4. AFM High-Resolution Contact Mode Image of HOPG (http://www.energosystems.ru/fgallery.htm)(http://www.energosystems.ru/fgallery.h

51 IV. Off-line Image Analysis. • Go to Off-line / View / Top View option and measure the spacings between atoms. The spacings should be as follows (

52 VI. References 1. Binnig, et al., Europhys. Lett. 3, 1281 (1987) [graphite] 2. Albrecht, et al., J. Vac. Sci. Tech. A 6 271 (1988) [molybdenum sul

53 14. TRAVEL AND REIMBURSMENT POLICIES Research grants will cover the following for approved business travel : 1. direct transportation (e.g. fli

6 Rua XV de Novembro, 1452 Caixa Postal 741 São Carlos, 13560-970, SP, Brasil [email protected] George Gluck (BIO-YR 4) ggluck RM 12-022 /

7 COLLABORATORS : Name Email (@mit.edu) Office / Work Phone (area code : 617) Phone (Home) B-DAY Collaborating Group Reuben Dormike rdomike 617 2

8 4. COLLABORATOR USER AGREEMENT AND POLICIES

9 Supervisor: Christine Ortiz, Assistant Professor Department of Materials Science and Engineering (DMSE) Room 13-4022, 77 Massachusetts Avenue Cam